DNA purification is a common and vital procedure in molecular biology. The aim of DNA purification is to separate the desired genetic material, chromosomal material from the contaminant (proteins, RNA and cell membrane). This is a vital procedure in nearly https://www.mpsciences.com all molecular applications. It must be done correctly to get high-quality, usable DNA.

There are many different approaches for DNA purification. The choice depends on a myriad of factors like the source materials, downstream applications, cost, and time constraints. The typical genomic and plasmid purification protocols require chemical treatment, enzymatic digestion or mechanical destruction of cells or tissues followed by salting out proteins and then precipitating the DNA with alcohol.

Ethanol precipitation is a simple cost-effective and quick method for desalting and concentration of DNA. DNA molecules form aggregates in the presence of monovalent cations like sodium and then precipitated from the solution by using the highest concentrations of alcohol. This method allows the removal of organic compounds, and other impurities from a sample. It is frequently used in conjunction with other purification techniques.

Anion exchange chromatography is yet another popular method of DNA purification. DNA in a solution is bound to positively charged resins via the interaction between the negatively charged DNA phosphate backbone as well as the positively charged surface molecules of the resin. During the binding steps the contaminants are removed using a stringent wash process. The purified DNA then is eluted under low salt conditions.